Mapping chromatin modifications at the single cell level
نویسندگان
چکیده
منابع مشابه
Simultaneously defining cell phenotypes, cell cycle, and chromatin modifications at single-cell resolution.
Heterogeneity of cellular phenotypes in asynchronous cell populations placed in the same biochemical and biophysical environment may depend on cell cycle and chromatin modifications; however, no current method can measure these properties at single-cell resolution simultaneously and in situ. Here, we develop, test, and validate a new microscopy assay that rapidly quantifies global acetylation o...
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Acute promyelocytic leukemia (APL) is characterized by specific chromosomal translocations, which generate fusion proteins such as promyelocytic leukemia (PML)-retinoic acid receptor (RAR) and promyelocytic leukemia zinc finger (PLZF)-RAR (X-RAR ). In this study, we have applied lac operator array systems to study the effects of X-RAR versus wild-type RAR on large-scale chromatin structure. The...
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One of the most rapidly advancing areas in genomics in recent years has been the explosion of platforms to assess properties at the single-cell level. The driving force behind these advancements has been a renaissance of sorts in how we think about the architecture and heterogeneity present in a population of cells (1, 2). While these concepts are not new, the advancement of technologies to ass...
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The timing of a cellular event often hides critical information on the process leading to the event. Our ability to measure event times in single cells along with other quantities allow us to learn about the drivers of the timed process and its downstream effects. In this review, we cover different types of events that have been timed in single cells, methods to time such events and types of an...
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A single cell creates surprising heterogeneity in a multicellular organism. While every organismal cell shares almost an identical genome, molecular interactions in cells alter the use of DNA sequences to modulate the gene of interest for specialization of cellular functions. Each cell gains a unique identity through molecular coding across the DNA, RNA, and protein conversions. On the other ha...
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ژورنال
عنوان ژورنال: Development
سال: 2019
ISSN: 1477-9129,0950-1991
DOI: 10.1242/dev.170217